Composite

Part:BBa_K4144082:Design

Designed by: Zhang Xinyan   Group: iGEM22_WHU-China   (2022-10-01)


a temperature switch that induces the express of EGFP below 36 ℃


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1686
    Illegal XhoI site found at 855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1158
    Illegal AgeI site found at 1504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2427


Design Notes

This composite is a temperature switch that induces the express of EGFP and makes the bacteria to show fluorescence under temperature of below 36 degree Celsius. TlpA36 is a temperature sensitive protein which is capable of repressing the pTlpA promoter under temperature of below 36 degree Celsius, thus repress the expression of PenI repressor. As a result, EFGP will express.


Source

This part comes from synthesis.

References